Sarkar A, Beard D A, Franza B R
Cell Systems Initiative, Bioengineering, University of Washington, Seattle, WA 98195-8070, USA.
Math Biosci. 2006 Jul;202(1):175-93. doi: 10.1016/j.mbs.2006.03.011. Epub 2006 Jun 13.
The effects of binding on the phosphorylation-dephosphorylation cycle (PDPC) - one of the key components of the signal transduction processes - is analyzed based on a mathematical model. The model shows that binding of proteins, forming a complex, diminishes the ultrasensitivity of the PDPC to the differences in activity between kinase and phosphatase in the cycle. It is also found that signal amplification depends upon the strength of the binding affinity of the protein (phosphorylated or dephosphorylated) to other proteins . It is also observed that the amplification of signal is not only dependent on phosphorylation potential but also on binding properties and resulting adjustments in binding energies.
基于一个数学模型，分析了结合对磷酸化-去磷酸化循环（PDPC）（信号转导过程的关键组成部分之一）的影响。该模型表明，形成复合物的蛋白质结合会降低PDPC对循环中激酶和磷酸酶活性差异的超敏感性。还发现信号放大取决于蛋白质（磷酸化或去磷酸化）与其他蛋白质结合亲和力的强度。还观察到信号放大不仅取决于磷酸化电位，还取决于结合特性以及由此产生的结合能调整。
