Hüttner E, Braun R, Wielgosz S M, Schöneich J
Institut für Genetik und Kulturpflanzenforschung, Gatersleben, Federal Republic of Germany.
Teratog Carcinog Mutagen. 1991;11(1):11-9. doi: 10.1002/tcm.1770110103.
The variant frequencies for 6-thioguanine-resistant spleen cells in different mouse strains have been estimated by autoradiography for animals without chemical treatment and in cases of in vivo mutagen dosage with ethylnitrosourea and cyclophosphamide, respectively. In untreated mice, the following variant frequencies have been found: C57Bl/6J, 2.84 x 10(-5);NMRI, 3.04 x 10(-5);DBA/2J, 5.91 x 10(-5). The selective concentration of 6-thioguanine was 100 microM for strains NMRI and DBA, while in the case of C57Bl with this concentration, no variant cells could be counted and a selective concentration of 50 microM was chosen. Treatment with 70, 140, and 210 mg/kg ethylnitrosourea resulted in increased variant frequencies in cells isolated 8 or 15 days later. On the other hand, doses of 20, 60, and 120 mg/kg cyclophosphamide did not result in a clear dose-response relationship of variant frequency in cells isolated 1, 8, 15, 22, and 29 days after treatment. These data are discussed with respect to findings in human populations exposed occupationally to cyclophosphamide.
通过放射自显影术，分别对未经化学处理的动物以及经体内亚硝基脲和环磷酰胺诱变剂量处理的动物，估算了不同小鼠品系中对6-硫鸟嘌呤耐药的脾细胞的变异频率。在未处理的小鼠中，发现了以下变异频率：C57Bl/6J为2.84×10⁻⁵；NMRI为3.04×10⁻⁵；DBA/2J为5.91×10⁻⁵。对于NMRI和DBA品系，6-硫鸟嘌呤的选择浓度为100微摩尔，而对于C57Bl品系，在此浓度下无法计数到变异细胞，因此选择了50微摩尔的选择浓度。用70、140和210毫克/千克亚硝基脲处理后，8天或15天后分离的细胞中变异频率增加。另一方面，20、60和120毫克/千克环磷酰胺的剂量在处理后1、8、15、22和29天分离的细胞中，并未导致变异频率呈现明显的剂量反应关系。结合职业接触环磷酰胺的人群的研究结果对这些数据进行了讨论。
