在环磷酰胺生产车间工作的工人外周血中，6-硫鸟嘌呤抗性淋巴细胞的频率增加。

Increased frequency of 6-thioguanine-resistant lymphocytes in peripheral blood of workers employed in cyclophosphamide production.

作者信息

Hüttner E, Mergner U, Braun R, Schöneich J

机构信息

Central Institute of Genetics and Research in Cultivated Plants, Academy of Sciences of the G.D.R., Gatersleben.

出版信息

Mutat Res. 1990 Feb;243(2):101-7. doi: 10.1016/0165-7992(90)90030-n.

DOI:10.1016/0165-7992(90)90030-n

PMID:2304480

Abstract

The frequency of 6-thioguanine-resistant peripheral blood lymphocytes has been determined by autoradiography in a control population and a population of cyclophosphamide-exposed individuals. The mean variant frequency in a non-exposed population was found to be 2.76 +/- 1.48 X 10(-5). Subpopulations of smokers and non-smokers revealed statistically significant differences in the variant frequencies, i.e. 3.52 +/- 1.55 X 10(-5) and 2.07 +/- 1.05 X 10(-5) respectively. In 20 out of a total of 23 individuals employed in cyclophosphamide synthesis and manufacturing, the variant frequency of 6-thioguanine-resistant lymphocytes was found to be higher than the maximum individual frequency found in the control population. The mean variant frequency in the cyclophosphamide-exposed population was 13.64 +/- 13.56 X 10(-5), a statistically significant increase as compared to the mean control frequency. There was no correlation between variant frequency and duration of employment suggesting that this test reflects the actual exposure and not a cumulative effect.

摘要

已通过放射自显影法测定了对照组人群和接触环磷酰胺人群中对6-硫鸟嘌呤耐药的外周血淋巴细胞的频率。在未接触人群中，平均变异频率为2.76±1.48×10⁻⁵。吸烟者和非吸烟者亚组的变异频率存在统计学上的显著差异，分别为3.52±1.55×10⁻⁵和2.07±1.05×10⁻⁵。在总共23名从事环磷酰胺合成和生产的人员中，有20人的6-硫鸟嘌呤耐药淋巴细胞变异频率高于对照组人群中发现的最高个体频率。接触环磷酰胺人群的平均变异频率为13.64±13.56×10⁻⁵，与对照组平均频率相比有统计学上的显著增加。变异频率与就业时长之间没有相关性，这表明该测试反映的是实际接触情况，而非累积效应。