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MARK2/EMK1/Par-1Balpha phosphorylation of Rab11-family interacting protein 2 is necessary for the timely establishment of polarity in Madin-Darby canine kidney cells.Rab11家族相互作用蛋白2的MARK2/EMK1/Par-1Bα磷酸化对于Madin-Darby犬肾细胞极性的及时建立是必要的。
Mol Biol Cell. 2006 Aug;17(8):3625-37. doi: 10.1091/mbc.e05-08-0736. Epub 2006 Jun 14.
2
Phosphorylation of Rab11-FIP2 regulates polarity in MDCK cells.Rab11-FIP2 的磷酸化调节 MDCK 细胞的极性。
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3
Rab11 family interacting protein 2 associates with Myosin Vb and regulates plasma membrane recycling.Rab11家族相互作用蛋白2与肌球蛋白Vb结合并调节质膜循环。
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Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition.磷酸化的Rab11-FIP2与Eps15的相互作用调节顶端连接复合体的组成。
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Rab11-FIP2 interaction with MYO5B regulates movement of Rab11a-containing recycling vesicles.Rab11-FIP2 与 MYO5B 的相互作用调节 Rab11a 包含的回收囊泡的运动。
Traffic. 2014 Mar;15(3):292-308. doi: 10.1111/tra.12146. Epub 2014 Jan 22.
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Identification and characterization of a family of Rab11-interacting proteins.Rab11相互作用蛋白家族的鉴定与特性分析。
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8
Rab11-FIP1 phosphorylation by MARK2 regulates polarity in MDCK cells.MARK2介导的Rab11-FIP1磷酸化调节MDCK细胞的极性。
Cell Logist. 2017 Jan 9;7(1):e1271498. doi: 10.1080/21592799.2016.1271498. eCollection 2017.
9
Rab11-family interacting protein 2 and myosin Vb are required for CXCR2 recycling and receptor-mediated chemotaxis.Rab11家族相互作用蛋白2和肌球蛋白Vb是CXCR2循环利用和受体介导的趋化作用所必需的。
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Rab11-FIP2 functions in transferrin recycling and associates with endosomal membranes via its COOH-terminal domain.Rab11-FIP2在转铁蛋白循环利用过程中发挥作用，并通过其羧基末端结构域与内体膜结合。
J Biol Chem. 2002 Jul 26;277(30):27193-9. doi: 10.1074/jbc.M200757200. Epub 2002 May 6.

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Rab11FIP1-deficient mice develop spontaneous inflammation and show increased susceptibility to colon damage.Rab11FIP1 缺陷小鼠发生自发性炎症，并表现出对结肠损伤的易感性增加。
Am J Physiol Gastrointest Liver Physiol. 2022 Sep 1;323(3):G239-G254. doi: 10.1152/ajpgi.00042.2022. Epub 2022 Jul 12.
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Overexpression of Rab11-FIP2 in colorectal cancer cells promotes tumor migration and angiogenesis through increasing secretion of PAI-1.Rab11-FIP2在结肠癌细胞中的过表达通过增加纤溶酶原激活物抑制因子-1的分泌促进肿瘤迁移和血管生成。
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9
MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity.MARK3 介导的 ARHGEF2 磷酸化将微管与肌动蛋白细胞骨架连接起来，从而建立细胞极性。
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The basolateral vesicle sorting machinery and basolateral proteins are recruited to the site of enteropathogenic E. coli microcolony growth at the apical membrane.基底外侧囊泡分选机制和基底外侧蛋白被募集到肠致病性大肠杆菌微菌落生长于顶端膜的部位。
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本文引用的文献

1
Assessment of Rab11-FIP2 interacting proteins in vitro.体外Rab11-FIP2相互作用蛋白的评估。
Methods Enzymol. 2005;403:706-15. doi: 10.1016/S0076-6879(05)03061-2.
2
E-cadherin-mediated adhesion is not the founding event of epithelial cell polarity in Drosophila.E-钙黏蛋白介导的黏附并非果蝇上皮细胞极性的起始事件。
Trends Cell Biol. 2005 May;15(5):237-40. doi: 10.1016/j.tcb.2005.03.001.
3
P-Mod: an algorithm and software to map modifications to peptide sequences using tandem MS data.P-Mod：一种利用串联质谱数据将修饰映射到肽序列的算法及软件。
J Proteome Res. 2005 Mar-Apr;4(2):358-68. doi: 10.1021/pr0498234.
4
Molecular perspective on tight-junction assembly and epithelial polarity.紧密连接组装与上皮极性的分子视角
Adv Drug Deliv Rev. 2005 Apr 25;57(6):815-55. doi: 10.1016/j.addr.2005.01.008.
5
Rab coupling protein is selectively degraded by calpain in a Ca2+-dependent manner.Rab偶联蛋白在钙离子依赖的方式下被钙蛋白酶选择性降解。
Biochem J. 2005 Jul 1;389(Pt 1):223-31. doi: 10.1042/BJ20042116.
6
Sample preparation and digestion for proteomic analyses using spin filters.使用离心过滤法进行蛋白质组学分析的样品制备与消化
Proteomics. 2005 May;5(7):1742-5. doi: 10.1002/pmic.200401063.
7
Rab11 in recycling endosomes regulates the sorting and basolateral transport of E-cadherin.循环内体中的Rab11调节E-钙黏蛋白的分选和基底外侧运输。
Mol Biol Cell. 2005 Apr;16(4):1744-55. doi: 10.1091/mbc.e04-10-0867. Epub 2005 Feb 2.
8
The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery.酵母的PAR-1同源物KIN1和KIN2与外排机制的组分存在遗传和物理相互作用。
Mol Biol Cell. 2005 Feb;16(2):532-49. doi: 10.1091/mbc.e04-07-0549. Epub 2004 Nov 24.
9
The C2 domains of the class I Rab11 family of interacting proteins target recycling vesicles to the plasma membrane.I类Rab11相互作用蛋白家族的C2结构域将回收囊泡靶向至质膜。
J Cell Sci. 2004 Sep 1;117(Pt 19):4365-75. doi: 10.1242/jcs.01280. Epub 2004 Aug 10.
10
Establishment and characterization of cultured epithelial cells lacking expression of ZO-1.缺乏紧密连接蛋白1（ZO-1）表达的培养上皮细胞的建立与特性分析
J Biol Chem. 2004 Oct 22;279(43):44785-94. doi: 10.1074/jbc.M406563200. Epub 2004 Jul 30.

Rab11家族相互作用蛋白2的MARK2/EMK1/Par-1Bα磷酸化对于Madin-Darby犬肾细胞极性的及时建立是必要的。

MARK2/EMK1/Par-1Balpha phosphorylation of Rab11-family interacting protein 2 is necessary for the timely establishment of polarity in Madin-Darby canine kidney cells.

作者信息

Ducharme Nicole A, Hales Chadwick M, Lapierre Lynne A, Ham Amy-Joan L, Oztan Asli, Apodaca Gerard, Goldenring James R

机构信息

Department of Surgery and Cell and Developmental Biology, Vanderbilt University School of Medicine, Vanderbilt-Ingram Cancer Center, and the Nashville VA Medical Center, Nashville, TN 37232, USA.

出版信息

Mol Biol Cell. 2006 Aug;17(8):3625-37. doi: 10.1091/mbc.e05-08-0736. Epub 2006 Jun 14.

DOI:10.1091/mbc.e05-08-0736
PMID:16775013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1525241/
Abstract

Rab11a, myosin Vb, and the Rab11-family interacting protein 2 (FIP2) regulate plasma membrane recycling in epithelial cells. This study sought to characterize more fully Rab11-FIP2 function by identifying kinase activities modifying Rab11-FIP2. We have found that gastric microsomal membrane extracts phosphorylate Rab11-FIP2 on serine 227. We identified the kinase that phosphorylated Rab11-FIP2 as MARK2/EMK1/Par-1Balpha (MARK2), and recombinant MARK2 phosphorylated Rab11-FIP2 only on serine 227. We created stable Madin-Darby canine kidney (MDCK) cell lines expressing enhanced green fluorescent protein-Rab11-FIP2 wild type or a nonphosphorylatable mutant [Rab11-FIP2(S227A)]. Analysis of these cell lines demonstrates a new role for Rab11-FIP2 in addition to that in the plasma membrane recycling system. In calcium switch assays, cells expressing Rab11-FIP2(S227A) showed a defect in the timely reestablishment of p120-containing junctional complexes. However, Rab11-FIP2(S227A) did not affect localization with recycling system components or the normal function of apical recycling and transcytosis pathways. These results indicate that phosphorylation of Rab11-FIP2 on serine 227 by MARK2 regulates an alternative pathway modulating the establishment of epithelial polarity.

摘要

Rab11a、肌球蛋白Vb和Rab11家族相互作用蛋白2（FIP2）调节上皮细胞中的质膜回收。本研究旨在通过鉴定修饰Rab11-FIP2的激酶活性来更全面地表征Rab11-FIP2的功能。我们发现胃微粒体膜提取物可使Rab11-FIP2的丝氨酸227位点发生磷酸化。我们鉴定出使Rab11-FIP2发生磷酸化的激酶为MARK2/EMK1/Par-1Bα（MARK2），并且重组MARK2仅使Rab11-FIP2的丝氨酸227位点发生磷酸化。我们构建了表达增强型绿色荧光蛋白-Rab11-FIP2野生型或不可磷酸化突变体[Rab11-FIP2(S227A)]的稳定的Madin-Darby犬肾（MDCK）细胞系。对这些细胞系的分析表明，Rab11-FIP2除了在质膜回收系统中发挥作用外，还具有新的功能。在钙转换实验中，表达Rab11-FIP2(S227A)的细胞在含p120的连接复合体的及时重建方面存在缺陷。然而，Rab11-FIP2(S227A)并不影响其与回收系统组分的定位，也不影响顶端回收和转胞吞途径的正常功能。这些结果表明，MARK2使Rab11-FIP2的丝氨酸227位点发生磷酸化，从而调节了一条调节上皮极性建立的替代途径。