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Rab11-FIP2在结肠癌细胞中的过表达通过增加纤溶酶原激活物抑制因子-1的分泌促进肿瘤迁移和血管生成。

Overexpression of Rab11-FIP2 in colorectal cancer cells promotes tumor migration and angiogenesis through increasing secretion of PAI-1.

作者信息

Dong Wenjie, Wu Xinai

机构信息

Department of Medical Oncology, the First Affiliated Hospital, Zhengzhou University, 1 East Jianshe Road, Zhengzhou, 450052 Henan People's Republic of China.

出版信息

Cancer Cell Int. 2018 Mar 9;18:35. doi: 10.1186/s12935-018-0532-0. eCollection 2018.

DOI:10.1186/s12935-018-0532-0
PMID:29540997
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5845176/
Abstract

BACKGROUND

Rab11 family-interacting protein 2 (Rab11-FIP2) can interact with MYO5B and plays an important role in regulating plasma membrane recycling. However, little is known about the clinical significance of DUSP2 in colorectal cancer (CRC).

METHODS

In this study, we investigated Rab11-FIP2 expression by immunohistochemistry in 125 patients with colorectal cancer. Conditioned media containing all secreted factors was harvested. Chemokine secretion and expression were analyzed by Chemi-array.

RESULTS

We found that the expression level of Rab11-FIP2 was significantly increased in colorectal cancer tissues and high expression of Rab11-FIP2 was closely correlated with nodal metastasis in colorectal cancer patients. Rab11-FIP2 overexpression promoted colorectal cancer metastasis in vitro and in vivo. Finally, we demonstrated that Rab11-FIP2 overexpression may contribute to increased secretion of PAI-1 in human colorectal cancer cells.

CONCLUSIONS

Our findings reveal a novel mechanism underlying the role of Rab11-FIP2 in colorectal cancer dissemination, suggesting that targeting Rab11-FIP2 might be a promising therapeutic strategy for CRC.

摘要

背景

Rab11家族相互作用蛋白2（Rab11-FIP2）可与MYO5B相互作用，并在调节质膜循环中发挥重要作用。然而，关于双特异性磷酸酶2（DUSP2）在结直肠癌（CRC）中的临床意义知之甚少。

方法

在本研究中，我们通过免疫组织化学检测了125例结直肠癌患者中Rab11-FIP2的表达情况。收集含有所有分泌因子的条件培养基。通过化学阵列分析趋化因子的分泌和表达。

结果

我们发现Rab11-FIP2在结直肠癌组织中的表达水平显著升高，且Rab11-FIP2的高表达与结直肠癌患者的淋巴结转移密切相关。Rab11-FIP2过表达在体外和体内均促进结直肠癌转移。最后，我们证明Rab11-FIP2过表达可能导致人结直肠癌细胞中纤溶酶原激活物抑制因子-1（PAI-1）分泌增加。

结论

我们的研究结果揭示了Rab11-FIP2在结直肠癌播散中作用的一种新机制，表明靶向Rab11-FIP2可能是一种有前景的CRC治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/de527d36ae08/12935_2018_532_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/72a26c89657c/12935_2018_532_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/89578ea2ee4a/12935_2018_532_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/e36a30ab598e/12935_2018_532_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/4997263145d8/12935_2018_532_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/bb3734d2d982/12935_2018_532_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/97ddc1fc888a/12935_2018_532_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/0f273f724777/12935_2018_532_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/6b9113340ea2/12935_2018_532_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/de527d36ae08/12935_2018_532_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/72a26c89657c/12935_2018_532_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/89578ea2ee4a/12935_2018_532_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/e36a30ab598e/12935_2018_532_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/4997263145d8/12935_2018_532_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/bb3734d2d982/12935_2018_532_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/97ddc1fc888a/12935_2018_532_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/0f273f724777/12935_2018_532_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/6b9113340ea2/12935_2018_532_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a48f/5845176/de527d36ae08/12935_2018_532_Fig9_HTML.jpg

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2
Proteomics analysis of differential protein expression identifies heat shock protein 47 as a predictive marker for lymph node metastasis in patients with colorectal cancer.差异蛋白质表达的蛋白质组学分析确定热休克蛋白47为结直肠癌患者淋巴结转移的预测标志物。
Int J Cancer. 2017 Mar 15;140(6):1425-1435. doi: 10.1002/ijc.30557.
3
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4
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Mol Ther Nucleic Acids. 2023 Jan 2;31:276-292. doi: 10.1016/j.omtn.2022.12.019. eCollection 2023 Mar 14.
5
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