Liu Wei, Pan Rui-Chi, Leng Jia-Yi, Ye Qing-Sheng
College of Life Sciences, South China Normal University, Guangzhou 510631, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Jun;32(3):369-74.
Effect of ABA on desiccation tolerance in the protocorm-like bodies (PLBs) of D. candidum was studied. The best result was obtained while ABA precultured at 5 micromol/L for 24 h (Fig.2). PLBs vitality rate was 10 times of the control after 2 h desiccation (Table 1). Detection of cytoplasmic osmoticums showed that ABA preculture could not induce the accumulations of soluble sugar, proline, etc (Tables 3, 4 and 7). But the content of soluble polysaccharide rose conspicuously (Table 5). So we think that the accumulation of soluble polysaccharide may play an important role in desiccation tolerance induced by ABA preculture.
研究了脱落酸(ABA)对铁皮石斛原球茎(PLBs)耐干燥性的影响。当ABA在5微摩尔/升的浓度下预培养24小时时,获得了最佳结果(图2)。干燥2小时后,原球茎的活力率是对照的10倍(表1)。细胞质渗透压的检测表明,ABA预培养不能诱导可溶性糖、脯氨酸等的积累(表3、4和7)。但可溶性多糖的含量显著上升(表5)。因此,我们认为可溶性多糖的积累可能在ABA预培养诱导的耐干燥性中起重要作用。