Jiang Shao-Tong, Wei Ming, Luo Jian-Ping
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
Sheng Wu Gong Cheng Xue Bao. 2006 Jul;22(4):613-8.
The effect of inorganic phosphate on cell growth, accumulation of polysaccharides together with nutrient utilization was investigated in suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100 g/L cells. The results indicate that the optimal concentration of phosphate in medium for cell growth of protocorm-like bodies of Dendrobium huoshanense was 2.5 mmol/L and biomass was 496.5 g/L (fresh weight). Phosphate was a limited factor for cell growth and there was relationship between the levels of intracellular phosphate and cell growth. 2.5 mmol/L of medium phosphate was beneficial to the absorption of carbohydrate and nitrate source. 0.312 mmol/L of medium phosphate was better for accumulation of polysaccharides and production of polysaccharides was 2.22 g/L at 36 d.
研究了无机磷酸盐对霍山石斛原球茎悬浮培养物中细胞生长、多糖积累及养分利用的影响。将30日龄的细胞以100 g/L细胞的接种密度转移至液体培养基中。结果表明，霍山石斛原球茎细胞生长的培养基中磷酸盐最佳浓度为2.5 mmol/L，生物量为496.5 g/L（鲜重）。磷酸盐是细胞生长的限制因素，细胞内磷酸盐水平与细胞生长之间存在关联。培养基中2.5 mmol/L的磷酸盐有利于碳水化合物和硝酸盐源的吸收。培养基中0.312 mmol/L的磷酸盐更有利于多糖积累，在36天时多糖产量为2.22 g/L。
