Rojas-Espinosa O, Silva-Miranda M, Wek-Rodriguez K, Arce-Paredes P
Department of Immunology, National School of Biological Sciences, National Polytechnic Institute, México D.F., México.
Scand J Clin Lab Invest. 2006;66(4):357-62. doi: 10.1080/00365510600672809.
We describe a technique designed to assess the optimal dilution of primary and secondary antibodies, to be used in Western blot, dot blot, the multi-antigen print immunoassay (MAPIA) and immunohistochemistry assays. The method that we call "line blot" is not an alternative but a practical, complementary tool for the above techniques that assures definitive results are obtained from single assays, so there is no need to repeat the assay. As with most immunoenzymatic assays, the line blot assay is very sensitive, allowing the detection of absolute amounts of antigen as low as 2.5 ng in the 0.5 cm-long segment line (see Results), depending on the strength of the secondary, enzyme-labelled antibody.
我们描述了一种旨在评估用于蛋白质免疫印迹、斑点印迹、多抗原印迹免疫分析（MAPIA）和免疫组织化学分析的一抗和二抗最佳稀释度的技术。我们称之为“线性印迹”的方法并非上述技术的替代方法，而是一种实用的补充工具，可确保从单次分析中获得明确结果，因此无需重复分析。与大多数免疫酶分析一样，线性印迹分析非常灵敏，根据二抗（酶标记抗体）的强度，能够检测到0.5厘米长的线性条带中低至2.5纳克的抗原绝对量（见结果）。
