Cui Wei, Wang Xin, Liu Yu-cun, Wan Yuan-lian, Guo Hong-jie, Zhu Jing
Department of General Surgery, Peking University First Hospital, BeiJing 100034, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Jun 18;38(3):280-3.
To investigate the relationship between HIC-5 and colonrectal cancer.
Samples were obtained from 42 patients with colonrectal cancer, and the mRNA and protein expressions of HIC-5/ARA55 were determined by RT-PCR and Western-blot respectively. FCM was used to investigate the apoptosis of Lovo in the HIC-5/ARA55 transfection cells. The expression of apoptosis related proteins Bax and Bcl-2 was determined by Western-blot, and the MTT assay was used to test cell growth speed affected by HIC-5/ARA55.
The expression of HIC-5 significantly decreased on colonrectal cancer tissues as compared with adjacent normal tissues on mRNA level and protein level(P<0.05). The apoptosis rate increase significantly in the HIC-5/ARA55 transfected cells. HIC-5/ARA55 could restrain tumor cell grow speed.
HIC-5/ARA55 is the protein that can reduce tumor cell growth rate, and induce apoptosis in colonrectal cancer cells.
研究HIC-5与结直肠癌的关系。
收集42例结直肠癌患者的样本，分别采用RT-PCR和Western-blot法检测HIC-5/ARA55的mRNA和蛋白表达。运用流式细胞术检测HIC-5/ARA55转染细胞中Lovo细胞的凋亡情况。采用Western-blot法检测凋亡相关蛋白Bax和Bcl-2的表达，并用MTT法检测HIC-5/ARA55对细胞生长速度的影响。
与癌旁正常组织相比，结直肠癌组织中HIC-5在mRNA水平和蛋白水平的表达均显著降低（P<0.05）。HIC-5/ARA55转染细胞的凋亡率显著增加。HIC-5/ARA55可抑制肿瘤细胞的生长速度。
HIC-5/ARA55是一种可降低肿瘤细胞生长速率并诱导结直肠癌细胞凋亡的蛋白。
