Semiautomated autologous bone marrow processing for transplantation.
作者信息
Pierelli L, Menichella G, Paoloni A, Serafini R, Vittori M, Foddai M L, Meoni F, Rossi P L, Leone G, Mango G
机构信息
Istituto di Semeiotica Medica, Università, Cattolica del Sacro Cuore, Rome, Italy.
出版信息
Haematologica. 1991 Mar;76 Suppl 1:10-1.
This paper describes the development of a semiautomated procedure for autologous bone marrow processing, prior to ex vivo manipulation and/or cryopreservation. This procedure was employed with a pediatric bowl (125 ml Latham bowl) and the automated DuPont Stericell processor. We have obtained a mononuclear cell recovery of 85% and a hemopoietic progenitor cell recovery of 81% (CFU-GM; BFU-E), with a red cell removal of 84%. We believe that a reliable and standardized bone marrow processing procedure is the basic necessity for a bone marrow transplantation program.
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