用于体外治疗和AB0血型不相容移植的骨髓单个核细胞浓缩：一种使用海莫奈蒂克斯V50细胞分离器的快速且可重复的方法。
Concentration of bone marrow mononuclear cells for in vitro treatment and AB0-incompatible transplantation: a rapid and reproducible procedure using the haemonetics V50 cell separator.
作者信息
Wiesneth M, Hertenstein B, Koerner K, Heimpel H, Heit W
机构信息
Internal Medicine III, University of Ulm, FRG.
出版信息
Beitr Infusionsther. 1988;21:340-5.
Forty-nine allogeneic and 14 autologous bone marrow grafts were processed with the Haemonetics V50 cell separator (Haemonetics Corp., Braintree, USA) for in vitro treatment with antibodies and cryopreservation respectively. The concentration of hemopoietic progenitor cells was performed without any sedimentation or density gradient agents. The recovery is given in percent (mean +/- sd) of the original marrow values: mononuclear cells (MNC) 74 +/- 10%, polymorphonuclear cells (PMC) 48 +/- 17%, red blood cells (RBC) 12 +/- 5%, granulocyte/monocyte progenitors (CFU-GM) 83 +/- 36%, and erythroid progenitors (BFU-E) 78 +/- 38%. The recovery of nucleated cells (NC) was 90 +/- 13% and the viability 82 +/- 11% after cryopreservation. The technique described provides a simple, rapid and efficient preparation of large bone marrow volumes for in vitro treatment and AB0-incompatible transplantation.
四十九份异体和十四份自体骨髓移植物分别使用美国布伦特里的海莫尼克斯公司生产的V50血细胞分离机进行处理，以便分别用抗体进行体外处理和冷冻保存。造血祖细胞浓度的测定未使用任何沉降剂或密度梯度剂。回收率以原始骨髓值的百分比（平均值±标准差）表示：单核细胞（MNC）74±10%，多形核细胞（PMC）48±17%，红细胞（RBC）12±5%，粒细胞/单核细胞祖细胞（CFU-GM）83±36%，以及红系祖细胞（BFU-E）78±38%。冷冻保存后有核细胞（NC）的回收率为90±13%，活力为82±11%。所描述的技术为体外处理和ABO血型不相容移植提供了一种简单、快速且高效的大体积骨髓制备方法。
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