卡氏肺孢子虫中的重复DNA。

Stringer S L, Hong S T, Giuntoli D, Stringer J R

Department of Molecular Genetics, Biochemistry, and Microbiology, College of Medicine, University of Cincinnati, Ohio 45267-0524.

J Clin Microbiol. 1991 Jun;29(6):1194-201. doi: 10.1128/jcm.29.6.1194-1201.1991.

A 16-kb DNA fragment designated Rp3-1 and cloned from the genome of rat-derived Pneumocystis carinii was found to contain sequences that were repeated approximately 70 times per genome. The repeated sequences in Rp3-1 spanned at least 10.4 kb. Sequences in Rp3-1 were present on each of the 16 P. carinii chromosomes resolved by field inversion gel electrophoresis. Most of the P. carinii genomic sequences homologous to those in the Rp3-1 clone appeared to be as long as those in the Rp3-1 clone but were highly polymorphic with respect to restriction enzyme cleavage sites. The Rp3-1 DNA fragment appears to be a member of a family of large, degenerate, dispersed repeats.

一个名为Rp3 - 1的16千碱基对DNA片段，从大鼠源性卡氏肺孢子虫基因组中克隆得到，发现其含有在每个基因组中大约重复70次的序列。Rp3 - 1中的重复序列跨度至少为10.4千碱基对。通过场反转凝胶电泳解析的16条卡氏肺孢子虫染色体上均存在Rp3 - 1中的序列。与Rp3 - 1克隆中的序列同源的大多数卡氏肺孢子虫基因组序列似乎与Rp3 - 1克隆中的序列一样长，但在限制性内切酶切割位点方面具有高度多态性。Rp3 - 1 DNA片段似乎是一个大型、退化、分散重复序列家族的成员。