大鼠卡氏肺孢子虫一种新型重复元件的分子特征分析

Molecular characterization of a novel repetitive element from Pneumocystis carinii from rats.

作者信息

Zhang J, Cushion M T, Stringer J R

机构信息

Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, College of Medicine, Ohio 45267-0560.

出版信息

J Clin Microbiol. 1993 Feb;31(2):244-8. doi: 10.1128/jcm.31.2.244-248.1993.

DOI:10.1128/jcm.31.2.244-248.1993

PMID:8432809

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262743/

Abstract

A repetitive DNA sequence, Rp-alpha, was isolated from rat-derived Pneumocystis carinii. The genome of rat-derived P. carinii contained 10 to 15 copies of Rp-alpha, which were located on most chromosomes, but no Rp-alpha could be detected in P. carinii derived from either humans or mice. Sequence analysis of two copies of the repeat showed them to be related but distinct. Each of them contained several copies of the 9-base sequence TAACCCTAA, arranged as direct repeats. Oligonucleotides consisting of multimers of this 9mer hybridized to the same set of chromosomes recognized by cloned copies of the Rp-alpha repeat. When used in DNA fingerprinting, the Rp-alpha repeat was capable of distinguishing between different isolates of rat-derived P. carinii.

摘要

从大鼠源性卡氏肺孢子虫中分离出一种重复DNA序列Rp-α。大鼠源性卡氏肺孢子虫的基因组含有10至15个Rp-α拷贝，这些拷贝位于大多数染色体上，但在人或小鼠源性的卡氏肺孢子虫中未检测到Rp-α。对两个重复拷贝的序列分析表明它们相关但不同。每个拷贝都包含几个9碱基序列TAACCCTAA的拷贝，排列成同向重复序列。由这个9聚体的多聚体组成的寡核苷酸与Rp-α重复序列的克隆拷贝识别的同一组染色体杂交。当用于DNA指纹分析时，Rp-α重复序列能够区分大鼠源性卡氏肺孢子虫的不同分离株。