Bhushan Ravi, Agarwal Rachna
Department of Chemistry, Indian Institute of Technology Roorkee, India.
Biomed Chromatogr. 2006 Jun-Jul;20(6-7):561-8. doi: 10.1002/bmc.650.
Arachin and its molecular species (arachin I and arachin II) were separated and isolated. The number and kind of subunits of arachin, arachin I and arachin II were determined. Studies were carried out under different experimental conditions using slab gel electrophoresis, size-exclusion chromatography and reversed-phase high-performance liquid chromatography. Gel electrophoresis was done under varying concentrations of resolving gel. Tube gel as well as slab gel electrophoresis were used and continuous as well as discontinuous buffer systems were used for both types of electrophoresis. In addition, the subunits were separated by reversed-phase HPLC using a gradient program. Arachin and arachin II were found to have 12 subunits each while arachin I showed six subunits. The subunits of arachin I were allowed to reconstitute by removing SDS. Eight combinations were tried for studying the reconstitution pattern. Molecular weight and weight ratio in each case were also determined.
花生球蛋白及其分子种类（花生球蛋白I和花生球蛋白II）被分离和提纯。测定了花生球蛋白、花生球蛋白I和花生球蛋白II的亚基数量和种类。在不同实验条件下，使用平板凝胶电泳、尺寸排阻色谱法和反相高效液相色谱法进行了研究。凝胶电泳在不同浓度的分离胶下进行。使用了管状凝胶以及平板凝胶电泳，并且两种类型的电泳都使用了连续和不连续缓冲系统。此外，通过使用梯度程序的反相高效液相色谱法分离亚基。发现花生球蛋白和花生球蛋白II各有12个亚基，而花生球蛋白I显示有6个亚基。通过去除十二烷基硫酸钠（SDS）使花生球蛋白I的亚基进行重组。尝试了八种组合来研究重组模式。还测定了每种情况下的分子量和重量比。
