通过离子交换色谱法与反相液相色谱法相结合制备大鼠肝脏的40S核糖体亚基蛋白。
Preparation of 40S ribosomal subunit proteins of rat liver by combination of ion exchange chromatography with reversed phase liquid chromatography.
作者信息
Dettmer R, Kärgel H J, Stahl J
出版信息
Biomed Biochim Acta. 1984;43(7):937-45.
PMID:6517889
Abstract
Total protein of small subunits of rat liver ribosomes was fractionated by chromatography on carboxymethylcellulose. Aliquots (between 5 and 30 mg) of the protein material of each peak were further separated by reversed phase liquid chromatography on an octadecasilyl silica column. In this way 15 of the 32 proteins of the small ribosomal subunit of rat liver could be isolated in milligram amounts.
摘要
大鼠肝脏核糖体小亚基的总蛋白通过羧甲基纤维素柱色谱进行分级分离。每个峰的蛋白质样品（5至30毫克）通过十八烷基硅烷硅胶柱上的反相液相色谱进一步分离。通过这种方法，大鼠肝脏核糖体小亚基的32种蛋白质中的15种能够以毫克量分离出来。
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