Noël J C, Paquin R, Ho-Kim M A, Tremblay R R
Biotechnol Appl Biochem. 1986 Apr-Jun;8(2-3):210-6.
Sciatin and transferrin are very similar glycoproteins which differ slightly in their carbohydrate content. In two-dimensional gel electrophoresis, they have one different isoform at pI lower than 5.77. However, highly different elution profiles have been recorded following size-exclusion HPLC. The use of a I-125 silica gel column has thus provided convincing evidence that both proteins do not show exactly the same hydrophobic three-dimensional structure.
坐骨神经素和转铁蛋白是非常相似的糖蛋白，它们的碳水化合物含量略有不同。在二维凝胶电泳中，它们在低于5.77的pI处有一个不同的异构体。然而，在尺寸排阻高效液相色谱后记录到了非常不同的洗脱曲线。因此，使用碘-125硅胶柱提供了令人信服的证据，表明这两种蛋白质的疏水三维结构并不完全相同。
