通过原位杂交检测胚胎组织和分层表皮中的基因表达。

Detection of gene expression in embryonic tissues and stratified epidermis by in situ hybridization.

作者信息

Morasso Maria I

机构信息

Developmental Skin Biology Section, NIH/NIAMS, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2010;585:253-60. doi: 10.1007/978-1-60761-380-0_17.

DOI:10.1007/978-1-60761-380-0_17

PMID:19908008

Abstract

Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.

摘要

尽管最近分子生物学的进展为确定局部基因表达提供了一种非常有效的方法，但通过对整装胚胎或石蜡包埋组织切片进行原位杂交来可视化基因表达，仍然是确定整体基因表达的一种出色方法。在本章中，我将回顾通过原位杂交技术确定mRNA表达的实验方案，特别关注发育中的复层表皮。