Zhu Zhi-tu, Jin Bo, Liu Yun-peng, Li Ying-chun, Lu Xiang-lan, Tian Xin, Hou Ke-zuo
Department of Medical Oncology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Nei Ke Za Zhi. 2006 Apr;45(4):314-7.
To investigate the effect of bufalin combined with all-trans retinoic acid-induced (ATRA) differentiation of acute promyelocytic leukemia (APL) cells in primary culture.
Fresh leukemia cells were obtained from heparinized bone marrow aspirations of 12 newly diagnosed APL patients. Cell viability was determined by trypan blue dye exclusion. Apoptosis of APL cell was assessed by morphological analysis. Differentiation of APL cell was also assessed by morphological analysis. Nitro blue tetrazolium (NBT) reduction test and expression of the granulocyte/macrophage-specific antigen CD(11)b was carried out with flow cytometric assay.
Bufalin combined with ATRA can induce differentiation of APL cells towards mature stages, NBT reduction was increased 15% - 52% and CD(11)b expression was also increased 16% - 69% in combination of bufalin and ATRA as compared with that of ATRA alone, while the concentration of ATRA needed in the combination group was reduced to 30% and the time of differentiation was reduced from 7 days to 4 days.
The combination of ATRA with bufalin can significantly enhance the differentiation of acute promyelocytic leukemia cells in primary culture by ATRA.
探讨蟾毒灵联合全反式维甲酸（ATRA）诱导原代培养的急性早幼粒细胞白血病（APL）细胞分化的作用。
从12例新诊断的APL患者经肝素抗凝的骨髓穿刺液中获取新鲜白血病细胞。通过台盼蓝拒染法测定细胞活力。通过形态学分析评估APL细胞的凋亡情况。同样通过形态学分析评估APL细胞的分化情况。采用硝基蓝四唑（NBT）还原试验及流式细胞术检测粒细胞/巨噬细胞特异性抗原CD(11)b的表达。
蟾毒灵联合ATRA可诱导APL细胞向成熟阶段分化，与单独使用ATRA相比，蟾毒灵与ATRA联合使用时NBT还原率提高了15% - 52%，CD(11)b表达也提高了16% - 69%，同时联合组所需的ATRA浓度降至30%，分化时间从7天缩短至4天。
ATRA与蟾毒灵联合应用可显著增强ATRA对原代培养的急性早幼粒细胞白血病细胞的分化作用。
