Miethbauer Sebastian, Haase Susann, Schmidtke Kai-Uwe, Günther Wolfgang, Heiser Ingrid, Liebermann Bernd
Friedrich-Schiller-Universität Jena, Institut für Pharmazie, Neugasse 23, D-07743 Jena, Germany.
Phytochemistry. 2006 Jun;67(12):1206-13. doi: 10.1016/j.phytochem.2006.05.003. Epub 2006 Jun 15.
Here we present the first isolation of the anthrachinone derivative rubellin A out of mycelium and culture filtrate of Ramularia collo-cygni. Furthermore, two compounds, rubellin E and 14-dehydro rubellin D were isolated and their structures elucidated. In comparison to the other rubellins, rubellin A shows increased photodynamic oxygen activation. By incorporating both [1-(13)C]-acetate and [2-(13)C]-acetate into the rubellins, we showed that such anthraquinone derivatives were biosynthesised via the polyketide pathway. The labelling pattern after being fed [U-(13)C(6)]-glucose proved the existence of fungal folding mode of the poly-beta-keto chain. The ability to produce rubellins is not limited to R. collo-cygni in the anamorph genus Ramularia.
在此，我们首次从柱隔孢属真菌（Ramularia collo-cygni）的菌丝体和培养滤液中分离出蒽醌衍生物红菌素A。此外，还分离出了两种化合物，红菌素E和14-脱氢红菌素D，并阐明了它们的结构。与其他红菌素相比，红菌素A表现出更强的光动力氧激活作用。通过将[1-(13)C]-乙酸盐和[2-(13)C]-乙酸盐掺入红菌素中，我们发现此类蒽醌衍生物是通过聚酮途径生物合成的。用[U-(13)C(6)]-葡萄糖喂养后的标记模式证明了聚-β-酮链的真菌折叠模式的存在。产生红菌素的能力并不局限于无性型柱隔孢属真菌中的柱隔孢菌。
