Nagamine Toshihiro, Kawasaki Yu, Matsumoto Shogo
RIKEN Discovery Research Institute, Wako-shi, Saitama 351-0198, Japan.
Virology. 2006 Sep 1;352(2):400-7. doi: 10.1016/j.virol.2006.04.034. Epub 2006 Jun 15.
Baculoviruses elicit the formation of a nuclear domain, called the virogenic stroma, in which viral DNA replication and nucleocapsid assembly occur. We had previously reported that nuclear focus formation of a transcriptional activator, IE1, is triggered by its binding to a viral DNA element, hr, and predicted that this hr-induced IE1 focus is an initial scaffold for the virogenic stroma. However, LEF3, a component of the virogenic stroma, did not localize to the IE1 foci. In exploring a mediator for its localization, we found that a baculovirus DNA helicase (P143), in combination with IE1 and hr, induced a subnuclear structure to which LEF3 localized and also that another component of the virogenic stroma, DBP, is able to localize to this structure. These results reveal that only four viral molecules are necessary to establish a nuclear domain which possesses a recruiting ability for a component of the virogenic stroma.
杆状病毒引发一种名为病毒发生基质的核结构域的形成，病毒DNA复制和核衣壳装配在其中发生。我们之前曾报道，转录激活因子IE1的核焦点形成是由其与病毒DNA元件hr结合触发的，并预测这种hr诱导的IE1焦点是病毒发生基质的初始支架。然而，病毒发生基质的一个组成部分LEF3并未定位于IE1焦点。在探索其定位的介导因子时，我们发现杆状病毒DNA解旋酶（P143）与IE1和hr一起，诱导了一个LEF3定位于其中的亚核结构，并且病毒发生基质的另一个组成部分DBP也能够定位于该结构。这些结果表明，仅需四个病毒分子就能建立一个对病毒发生基质的一个组成部分具有招募能力的核结构域。
