RIKEN, Wako-shi, Saitama, Japan.
RIKEN, Wako-shi, Saitama, Japan.
Virus Res. 2015 Oct 2;208:120-8. doi: 10.1016/j.virusres.2015.06.005. Epub 2015 Jun 15.
Prior to viral DNA replication, baculovirus IE1 exhibits a focal distribution within the cell nucleus. During DNA replication, the IE1 foci apparently expand and develop into a virus replication center called the virogenic stroma (VS). In our search for chemical compounds capable of modulating Bombyx mori nucleopolyhedrovirus (BmNPV: a prototype of baculovirus) replication, we found an inhibitor (dBIQdO) of IE1 focus formation. VS formation, however, was not affected, suggesting that IE1 foci are not essential for VS formation and that IE1 possesses two independent mechanisms for sub-nuclear localization. In addition to inhibition of IE1 focus formation, dBIQdO also reduced viral titers following infection at a low MOI. Comparison of the effects of three chemicals, dBIQdO, aphidicolin and caffeine, on IE1 localization allowed us to detect a shift from focal distribution to VS localization, suggesting that IE1 foci are disassembled prior to VS formation.
在病毒 DNA 复制之前，杆状病毒 IE1 在细胞核内呈点状分布。在 DNA 复制过程中，IE1 焦点明显扩大，并发展成称为病毒发生基质（VS）的病毒复制中心。在寻找能够调节家蚕核型多角体病毒（BmNPV：杆状病毒的原型）复制的化学化合物的过程中，我们发现了一种 IE1 焦点形成的抑制剂（dBIQdO）。然而，VS 的形成并没有受到影响，这表明 IE1 焦点对于 VS 的形成不是必需的，并且 IE1 具有两个独立的亚核定位机制。除了抑制 IE1 焦点的形成外，dBIQdO 还在低 MOI 感染时降低了病毒滴度。比较三种化学物质（dBIQdO、阿非迪可林和咖啡因）对 IE1 定位的影响，我们可以发现从点状分布到 VS 定位的转变，这表明在 VS 形成之前，IE1 焦点被分解。
