Tohda Shuji, Sato Taku, Kogoshi Hanae, Fu Lu, Sakano Seiji, Nara Nobuo
Department of Laboratory Medicine, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-Ku, Tokyo 113-8519, Japan.
Leuk Res. 2006 Nov;30(11):1385-90. doi: 10.1016/j.leukres.2006.05.003. Epub 2006 Jun 14.
A novel lymphoma cell line, designated TMD8 was established from cells of a patient with diffuse large B-cell lymphoma. TMD8 cells expressed HES1 mRNA, suggesting constitutive activation of Notch signaling. TMD8 cells expressed normal-sized Notch1 protein, and showed no mutations in the NOTCH1 gene. Cell growth was suppressed by gamma-secretase inhibitors (GSI). It was reported that GSI suppressed growth of T-cell acute lymphoblastic leukemia (T-ALL) cell lines, which frequently had NOTCH1 mutations. In addition to T-ALL, TMD8 is another unique cell line sensitive to GSI, and is useful to study effects of GSI in molecular targeting therapy.
一种名为TMD8的新型淋巴瘤细胞系是从一名弥漫性大B细胞淋巴瘤患者的细胞中建立的。TMD8细胞表达HES1 mRNA，提示Notch信号通路的组成性激活。TMD8细胞表达正常大小的Notch1蛋白，且NOTCH1基因未发生突变。γ-分泌酶抑制剂（GSI）可抑制细胞生长。据报道，GSI可抑制经常发生NOTCH1突变的T细胞急性淋巴细胞白血病（T-ALL）细胞系的生长。除T-ALL外，TMD8是另一种对GSI敏感的独特细胞系，有助于研究GSI在分子靶向治疗中的作用。
