离子液体改进的“绿色”色谱法测定柑橘类草药中的章鱼胺、辛弗林和酪胺

Determination of octopamine, synephrine and tyramine in Citrus herbs by ionic liquid improved 'green' chromatography.

作者信息

Tang Fei, Tao Liang, Luo Xubiao, Ding Li, Guo Manli, Nie Lihua, Yao Shouzhuo

机构信息

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.

出版信息

J Chromatogr A. 2006 Sep 1;1125(2):182-8. doi: 10.1016/j.chroma.2006.05.049. Epub 2006 Jun 16.

DOI:10.1016/j.chroma.2006.05.049

PMID:16781718

Abstract

Without adding any volatile organic solvents, aqueous solutions of room temperature ionic liquids (RTILs) were used as 'green' mobile phases to determine octopamine, synephrine and tyramine by liquid chromatography. The problems of the adrenergic amines separation, such as band tailing, low retention and low resolution were solved successfully by using RTIL. The effect of 1-ethyl-3-methylimidazolium tertafluoroborate ([EMIM][BF4]) was the best in the six investigated RTILs. The concentration of [EMIM][BF4], mobile phase pH and column temperature, which influenced the chromatographic behaviors of the analytes, were investigated in detail. The change of retention factors caused by pH shift was obviously suppressed by [EMIM][BF4]. The sensitivity, accuracy and repeatability of this method were found to be satisfactory. The contents of adrenergic amines in several Citrus herbs and extracts, such as Fructus aurantii immaturus, were simultaneously determined by this 'green' chromatographic method.

摘要

在不添加任何挥发性有机溶剂的情况下，室温离子液体（RTILs）的水溶液被用作“绿色”流动相，通过液相色谱法测定章鱼胺、辛弗林和酪胺。使用RTIL成功解决了肾上腺素能胺类分离中的拖尾、保留时间短和分离度低等问题。在六种研究的RTILs中，1-乙基-3-甲基咪唑四氟硼酸盐（[EMIM][BF4]）的效果最佳。详细研究了[EMIM][BF4]的浓度、流动相pH值和柱温对分析物色谱行为的影响。[EMIM][BF4]明显抑制了pH值变化引起的保留因子变化。该方法的灵敏度、准确性和重复性令人满意。通过这种“绿色”色谱方法同时测定了几种柑橘类草药和提取物（如枳实）中肾上腺素能胺类的含量。