高效液相色谱法测定青皮中辛弗林和N-甲基酪胺的含量
[Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC].
作者信息
Li Xian-duan, Ma Zhi-jing, Lin Sheng, Gu Xue-zhu, Mao Shu-jie
机构信息
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2004 Jun;29(6):537-9.
OBJECTIVE
To establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.
METHOD
Samples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.1) as mobile phase. Detection wavelength was 285 nm.
RESULT
Synephrine and N-methyltyramine in sample solution were well separated. Linearity of synephrine was good (r = 0.9999) in range of 0.35-11.24 microg. The average recovery was 97.1%, and RSD of repeatability was 1.9%.
CONCLUSION
This method can be used for quality control of Citri Reticulatae.
目的
建立测定陈皮中辛弗林和N - 甲基酪胺含量的定量方法。
方法
样品用30%甲醇提取。采用ODS柱，以甲醇 - 水 - 十二烷基硫酸钠（55:45:0.1）为流动相。检测波长为285 nm。
结果
样品溶液中的辛弗林和N - 甲基酪胺得到良好分离。辛弗林在0.35 - 11.24μg范围内线性良好（r = 0.9999）。平均回收率为97.1%，重复性RSD为1.9%。
结论
该方法可用于陈皮的质量控制。
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