Putzbach Karsten, Rimmer Catherine A, Sharpless Katherine E, Sander Lane C
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
J Chromatogr A. 2007 Jul 13;1156(1-2):304-11. doi: 10.1016/j.chroma.2006.12.027. Epub 2007 May 23.
Four adrenergic amines [synephrine, octopamine, tyramine, and n-methyltyramine] were determined in a variety of Bitter Orange containing dietary supplements. Two extraction techniques were evaluated in detail: Soxhlet extraction and sonication extraction. A liquid chromatographic separation using a reversed-phase C(18) stationary phase and the ion-pairing reagent sodium dodecyl sulfate was developed to separate the Bitter Orange alkaloids. Ultraviolet absorbance detection at 220 nm and fluorescence detection with excitation at 273 nm and emission at 304 nm were used for the alkaloid detection. The method described was used for the assignment of the levels of the predominant alkaloids in three candidate standard reference materials containing Bitter Orange.
在多种含苦橙的膳食补充剂中测定了四种肾上腺素能胺类物质[辛弗林、章鱼胺、酪胺和N - 甲基酪胺]。详细评估了两种提取技术：索氏提取法和超声提取法。开发了一种使用反相C(18)固定相和离子对试剂十二烷基硫酸钠的液相色谱分离方法，用于分离苦橙生物碱。采用220 nm处的紫外吸光度检测以及激发波长为273 nm、发射波长为304 nm的荧光检测来测定生物碱。所描述的方法用于确定三种含苦橙的候选标准参考物质中主要生物碱的含量。
