Keles Gonca, Basoglu Tarik, Yapici Oktay, Cetinkaya Burcu Ozkan, Acikgoz Gokhan, Firatli Erhan
Ondokuzmayis University, Faculty of Dentistry, Department of Periodontology, Samsun, Istanbul, Turkey.
Indian J Med Res. 2006 Apr;123(4):541-6.
BACKGROUND & OBJECTIVE: It is not clear how lathyrism affects the systemic bone metabolism. We therefore undertook a study to observe periodontal and systemic bone changes by performing radiological, metabolic, and bone densitometric evaluations in rats with experimental lathyrism.
A total of 30 rats were used. Experimental lathyrism was induced by once daily subcutaneous administration of beta-aminopropionitrile (beta-APN), at a dose of 5 mg beta-APN/0.4 ml per 100 g of body weight for 40 days. After 40 days, vertebral bone mineral density was analyzed by means of dual energy X-ray absorbtiometry in both groups. Blood was drawn by cardiac puncture and the animals were decapitated. Serum calcium levels were measured. Right mandibles were removed and radiographs were obtained. Alveolar bone level was determined in the radiographs.
In all lathyritic rats, alveolar bone level was pathologically decreased with visible resorption. Vertebral bone mineral density values of lathyritic rats did not differ significantly from those of the control group. Compared to controls, there was a statistically significant decrease in serum calcium levels in the lathyritic group (P<0.001).
INTERPRETATION & CONCLUSION: Significant alveolar bone resorption without alterations in vertebral bone mineral density indicated that lathyrogen administration for 40 days presumably has not caused systemic demineralization. This model could be used for studying the role of local and systemic agents on periodontal alveolar bone resorption.
尚不清楚山黧豆中毒如何影响全身骨骼代谢。因此，我们开展了一项研究，通过对实验性山黧豆中毒大鼠进行放射学、代谢和骨密度评估，观察牙周和全身骨骼的变化。
共使用30只大鼠。通过每天皮下注射一次β-氨基丙腈（β-APN）诱导实验性山黧豆中毒，剂量为每100克体重5毫克β-APN/0.4毫升，持续40天。40天后，两组均采用双能X线吸收法分析椎骨骨密度。通过心脏穿刺采血，然后将动物断头。测量血清钙水平。取出右侧下颌骨并拍摄X线片。在X线片中确定牙槽骨水平。
在所有山黧豆中毒大鼠中，牙槽骨水平出现病理性降低并有明显吸收。山黧豆中毒大鼠的椎骨骨密度值与对照组相比无显著差异。与对照组相比，山黧豆中毒组血清钙水平有统计学意义的降低（P<0.001）。
牙槽骨明显吸收但椎骨骨密度无改变，表明给予山黧豆毒素40天可能未引起全身脱矿质作用。该模型可用于研究局部和全身因素在牙周牙槽骨吸收中的作用。
