Oral Pathology Department, Faculty of Oral & Dental Medicine, Cairo University, Cairo, Egypt.
J Oral Pathol Med. 2010 May;39(5):424-30. doi: 10.1111/j.1600-0714.2009.00877.x. Epub 2010 Feb 24.
The present study was designed to study the effect of beta-aminopropionitrile (beta-APN), present in Lathyrus sativus grass pea consumed in drought prone areas, on dentin of the continuously erupting rat incisors.
Eighteen adult male rats were used. In the experimental group (18 rats), lathyrism was induced by a once daily subcutaneous administration of beta-APN for 40 days. The maxillary and mandibular incisors were examined ultrastructurally and morphometrically.
The mean number of patent tubules, the mean area, perimeter and the area percent of the tubules were analyzed. Ultrastructurally, the dentinal tubules of both coronal and radicular dentin in the lathyritic group were narrower or even obliterated compared with those in the control. The coronal and radicular dentin of the lathyritic group exhibited an irregular lattice of non-mineralized small branching collagen fibrils obliterating the dentinal tubules. The mean number of patent tubules in the control and lathyritic groups revealed an insignificant difference. The mean area of the tubules showed a statistically significant difference in lathyritic radicular dentin (P = 0.0353). The percentage of the total surface area of the dentinal tubules significantly decreased in the radicular dentin of the lathyritic group (P = 0.024).
These findings indicated a deleterious effect of lathyrism on dentin, with a possible negative impact on developing teeth integrity.
本研究旨在研究在干旱地区食用的兵豆草中的β-氨基丙腈（β-APN）对持续萌出的大鼠切牙牙本质的影响。
使用 18 只成年雄性大鼠。在实验组（18 只大鼠）中，通过每日一次皮下给予β-APN 40 天来诱导豆病。对上颌和下颌切牙进行超微结构和形态计量学检查。
分析了有髓小管的平均数量、平均面积、周长和小管的面积百分比。超微结构上，与对照组相比，病变组的冠根牙本质小管较窄甚至闭塞。病变组的冠根牙本质表现为非矿化的小分支胶原纤维不规则晶格，闭塞牙本质小管。对照组和病变组有髓小管的平均数量无显著差异。小管的平均面积在病变组的根牙本质中表现出统计学上的显著差异（P = 0.0353）。病变组根牙本质中牙本质小管的总表面积百分比明显下降（P = 0.024）。
这些发现表明豆病对牙本质有有害影响，可能对发育中牙齿的完整性产生负面影响。
