Volpe B T, Binder H J
Biochim Biophys Acta. 1975 Jul 18;394(4):597-604. doi: 10.1016/0005-2736(75)90145-5.
The effect of conjugated dihydroxy and trihydroxy bile salts on electrolyte transport across isolated rabbit jejunal mucosa was studied. Both taurochenodeoxycholic acid and taurocholic acid increased the short-circuit current (Isc) in bicarbonate-Ringer solution but not in a bicarbonate-free, chloride-free solution. Taurochenodeoxycholic acid was significantly more effective than taurocholic acid in increasing Isc. The presence of theophylline prevented the taurochenodeoxycholic acid- and taurocholic acid-induced increase in Isc. Transmural ion fluxes across jejunal mucosa demonstrated that 2 mM taurochenodeoxycholic acid decreased net Na+ absorption, increased net Cl- secretion and increased the residual flux (which probably represents HCO3- secretion). These studies support the hypothesis that cyclic AMP may be a mediator of intestinal electrolyte secretion.
研究了共轭二羟基和三羟基胆汁盐对兔空肠黏膜电解质转运的影响。牛磺鹅去氧胆酸和牛磺胆酸在碳酸氢盐 - 林格液中均能增加短路电流（Isc），但在无碳酸氢盐、无氯化物的溶液中则不能。在增加Isc方面，牛磺鹅去氧胆酸比牛磺胆酸显著更有效。茶碱的存在可阻止牛磺鹅去氧胆酸和牛磺胆酸诱导的Isc增加。空肠黏膜的跨壁离子通量表明，2 mM牛磺鹅去氧胆酸可减少净Na⁺吸收、增加净Cl⁻分泌并增加残余通量（可能代表HCO₃⁻分泌）。这些研究支持环磷酸腺苷（cAMP）可能是肠道电解质分泌介质的假说。
