Gonzalez C A, Rees K R
Biochim Biophys Acta. 1975 Jul 7;395(3):361-72. doi: 10.1016/0005-2787(75)90208-7.
A peak in the non-histone chromosomal protein polyacrylamide gel electrophoresis profiles has been detected which is higher in log phase 3T3 and 3T3/SV40 cells than in density-inhibited 3T3 cells. Radioactive incorporation is substantially higher into this peak in log phase 3T3 than in 3T3/SV40 and density-inhibited 3T3 cells. Reversion of 3T3/SV40 cells with dibutyryl cyclic AMP and theophylline produces increased radioactive incorporation into the peak. Electrophoresis of non-histone chromosomal proteins extracted at different stages of the cell cycle in density inhibited 3T3 cells following serum stimulation shows a cyclic variation in the amount of this peak with maximum accumulation in late G1. In contrast the height of an equivalent peak in synchronously growing 3T3/SV40 cells remains constant throughout the cell cycle. It is postulated that the protein(s) of this peak may have a regulatory role in cell growth.
在非组蛋白染色体蛋白质聚丙烯酰胺凝胶电泳图谱中检测到一个峰值，该峰值在对数期的3T3和3T3/SV40细胞中比在密度抑制的3T3细胞中更高。对数期3T3细胞中该峰值的放射性掺入量比3T3/SV40细胞和密度抑制的3T3细胞中高得多。用二丁酰环磷腺苷和茶碱使3T3/SV40细胞回复，会使该峰值的放射性掺入增加。在血清刺激后，对密度抑制的3T3细胞在细胞周期不同阶段提取的非组蛋白染色体蛋白质进行电泳，结果显示该峰值的量呈周期性变化，在G1晚期积累最多。相比之下，同步生长的3T3/SV40细胞中同等峰值的高度在整个细胞周期中保持恒定。据推测，该峰值的蛋白质可能在细胞生长中具有调节作用。
