Houghton R L, Fisher R J, Sanadi D R
Biochim Biophys Acta. 1975 Jul 8;396(1):17-23. doi: 10.1016/0005-2728(75)90185-1.
Active transport vesicles of Escherichia coli were shown to possess low levels of energy-independent and energy-dependent nicotinamide nucleotide transhydrogenase activities. Breakage of such vesicles in a French pressure cell resulted in a fraction which had an 8-10-fold increased respiration- and ATP-driven transhydrogenase activities. Stimulation of the ATPase activity in vesicles with Triton X-100 was also paralledled by a 2-fold increase in the energy-independent transhydrogenase. Disruption of the vesicles similarly resulted in increases in the energy-independent transhydrogenase, NADH and succinate oxidase activities but a decrease in succinate supported proline uptake. In the light of these findings, the "sidedness' of the vesicle membranes is discussed.
已表明大肠杆菌的主动运输囊泡具有低水平的非能量依赖性和能量依赖性烟酰胺核苷酸转氢酶活性。在法国压力室中破碎此类囊泡会产生一部分，其呼吸和ATP驱动的转氢酶活性增加了8至10倍。用Triton X-100刺激囊泡中的ATP酶活性，非能量依赖性转氢酶也会相应增加2倍。类似地，囊泡的破裂导致非能量依赖性转氢酶、NADH和琥珀酸氧化酶活性增加，但琥珀酸支持的脯氨酸摄取减少。根据这些发现，讨论了囊泡膜的“不对称性”。
