Malendowicz Ludwik K, Trejter Marcin, Rebuffat Piera, Ziolkowska Agnieska, Nussdorfer Gastone G, Majchrzak Mariola
Department of Histology and Embryology, School of Medicine, PL-60781 Poznan, Poland.
Int J Mol Med. 2006 Jul;18(1):197-200.
The effects of some endocrine disruptors that possess estrogen-like activity on the secretion and growth of regenerating rat adrenal cortex have been investigated in ovariectomized (OVX) and sham-OVX rats. As reference groups, dexamethasone (Dx)-administered sham-OVX and 17beta-estradiol-administered OVX animals were used. Dx, estradiol and endocrine disruptors were subcutaneously injected daily at a dose of 3 nmoles/100 g for 10 consecutive days after surgery, and adrenal enucleation was performed on day 5 of the experiment. Dx and genistein significantly decreased corticosterone plasma concentration (as measured by RIA) in sham-OVX rats with regenerating adrenals, while other disruptors (eusolex, procymidone, linurone, resveratrol, bisphenol-A and and silymarin) were ineffective. Mitotic index (as assayed by the stachmokinetic method with vincristine) was not changed by either Dx or disruptors. Estradiol significantly increased and genistein significantly lowered corticosterone blood level in OVX rats; similar effects were induced in the mitotic index of regenerating adrenals, but the changes were not significant. Eusolex increased the mitotic index, without altering the level of circulating corticosterone. Collectively, our findings allow us to conclude that, of the endocrine disruptors tested, only genistein is able to suppress the secretory activity of regenerating adrenal cortex, this Dx-like effect being apparently unrelated to its estrogen-like activity, and only eusolex enhances the proliferation rate of regenerating adrenal, the effect being conceivably connected with its estrogen-like activity.
在去卵巢（OVX）大鼠和假去卵巢大鼠中，研究了一些具有雌激素样活性的内分泌干扰物对再生大鼠肾上腺皮质分泌和生长的影响。作为参照组，使用了给予地塞米松（Dx）的假去卵巢大鼠和给予17β-雌二醇的去卵巢动物。手术后连续10天，每天以3纳摩尔/100克的剂量皮下注射Dx、雌二醇和内分泌干扰物，在实验第5天进行肾上腺摘除术。Dx和染料木黄酮显著降低了具有再生肾上腺的假去卵巢大鼠的皮质酮血浆浓度（通过放射免疫分析法测定），而其他干扰物（依索唑胺、腐霉利、利谷隆、白藜芦醇、双酚A和水飞蓟宾）则无效。有丝分裂指数（通过用长春新碱的细胞分裂动力学方法测定）未因Dx或干扰物而改变。雌二醇显著升高而去卵巢大鼠的皮质酮血水平，染料木黄酮则显著降低；对再生肾上腺的有丝分裂指数也有类似影响，但变化不显著。依索唑胺增加了有丝分裂指数，而未改变循环皮质酮水平。总体而言，我们的研究结果使我们能够得出结论，在所测试的内分泌干扰物中，只有染料木黄酮能够抑制再生肾上腺皮质的分泌活性，这种类似Dx的作用显然与其雌激素样活性无关，只有依索唑胺提高了再生肾上腺的增殖率，这种作用可能与其雌激素样活性有关。
