Zafra-Gómez Alberto, Garballo Antonio, Morales Juan C, García-Ayuso Luis E
Quality Department, Analytical Division, Puleva Biotech S.A., Camino de Purchil 66, E-18004 Granada, Spain.
J Agric Food Chem. 2006 Jun 28;54(13):4531-6. doi: 10.1021/jf060346e.
A fast, simple, and reliable method for the isolation and determination of the vitamins thiamin, riboflavin, niacin, pantothenic acid, pyridoxine, folic acid, cyanocobalamin, and ascorbic acid in food samples is proposed. The most relevant advantages of the proposed method are the simultaneous determination of the eight more common vitamins in enriched food products and a reduction of the time required for quantitative extraction, because the method consists merely of the addition of a precipitation solution and centrifugation of the sample. Furthermore, this method saves a substantial amount of reagents as compared with official methods, and minimal sample manipulation is achieved due to the few steps required. The chromatographic separation is carried out on a reverse phase C18 column, and the vitamins are detected at different wavelengths by either fluorescence or UV-visible detection. The proposed method was applied to the determination of water-soluble vitamins in supplemented milk, infant nutrition products, and milk powder certified reference material (CRM 421, BCR) with recoveries ranging from 90 to 100%.
本文提出了一种快速、简单且可靠的方法，用于分离和测定食品样品中的硫胺素、核黄素、烟酸、泛酸、吡哆醇、叶酸、氰钴胺素和抗坏血酸等维生素。该方法最显著的优点是能够同时测定强化食品中八种最常见的维生素，并减少了定量提取所需的时间，因为该方法仅需加入沉淀溶液并对样品进行离心。此外，与官方方法相比，该方法节省了大量试剂，且由于所需步骤较少，实现了最小化的样品处理。色谱分离在反相C18柱上进行，维生素通过荧光或紫外可见检测在不同波长下进行检测。该方法应用于测定强化牛奶、婴儿营养产品和奶粉标准参考物质（CRM 421，BCR）中的水溶性维生素，回收率在90%至100%之间。
