Nguyen Quang Huy, Hoang Anh Quoc, Truong Thi My Hanh, Dinh Thi Diu, Le Thi Thuy, Luu Thi Huyen Trang, Dinh Viet Chien, Nguyen Thi Minh Thu, Vu Thi Trang, Nguyen Thi Anh Huong
Faculty of Chemistry, University of Science, Vietnam National University, 19 Le Thanh Tong, Hanoi 10000, Vietnam.
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Thai Nguyen University of Medicine and Pharmacy, Thai Nguyen 24000, Vietnam.
J Anal Methods Chem. 2021 May 5;2021:5526882. doi: 10.1155/2021/5526882. eCollection 2021.
A method for the simultaneous determination of seven B-group vitamers including thiamine, riboflavin, nicotinamide, niacin, pyridoxine, pyridoxal, and pyridoxamine in nutritional products by using enzymatic digestion followed by LC-MS/MS quantification was studied. The LC-MS/MS conditions such as MS transitions, mobile phase programs, and ammonium formate buffer concentrations, and sample treatment procedures (e.g., concentrations of buffer solution, digestion temperature, and digestion time) were investigated. The analytical method performance was evaluated by multiple criteria such as selectivity, linearity, detection and quantification limits, repeatability, reproducibility, and recovery by using real sample matrices. The validated method was successfully applied to analyze vitamin B concentrations in different nutritional products like ultra-heat-treated milk, powdered milk, and nutritional powder. Vitamin B concentrations varied over a wide range from lower than detection limits to about 9000 g/100 g, depending on vitamin groups, compound forms, and sample types. The measured concentrations of B-group vitamins in our samples were generally in good agreement with values of label claims.
研究了一种通过酶消化后采用液相色谱-串联质谱（LC-MS/MS）定量法同时测定营养产品中硫胺素、核黄素、烟酰胺、烟酸、吡哆醇、吡哆醛和吡哆胺七种B族维生素的方法。研究了LC-MS/MS条件，如质谱转换、流动相程序、甲酸铵缓冲液浓度，以及样品处理程序（如缓冲溶液浓度、消化温度和消化时间）。使用实际样品基质，通过选择性、线性、检测限和定量限、重复性、再现性和回收率等多个标准对分析方法性能进行了评估。该验证方法成功应用于分析不同营养产品（如超高温处理牛奶、奶粉和营养粉）中的维生素B浓度。根据维生素种类、化合物形式和样品类型，维生素B浓度变化范围很广，从低于检测限到约9000μg/100g。我们样品中测得的B族维生素浓度通常与标签声明值高度一致。
