Kim Kwan Su, Park Si Hyung, Choung Myoung Gun
Department of Medicinal Plant Resources, Mokpo National University, Muan 534-729, Korea.
J Agric Food Chem. 2006 Jun 28;54(13):4544-50. doi: 10.1021/jf0605603.
Sesame (Sesamum indicum L.) contains abundant lignans including lipid-soluble lignans (sesamin and sesamolin) and water-soluble lignan glycosides (sesaminol triglucoside and sesaminol diglucoside) related to antioxidative activity. In this study, near infrared reflectance spectroscopy (NIRS) was used to develop a rapid and nondestructive method for the determination of lignan contents on intact sesame seeds. Ninety-three intact seeds were scanned in the reflectance mode of a scanning monochromator. This scanning procedure did not require the pulverization of samples, allowing each analysis to be completed within minutes. Reference values for lignan contents were obtained by high-performance liquid chromatography analysis. Calibration equations for lignans (sesamin and sesamolin) and lignan glycosides (sesaminol triglucoside and sesaminol diglucoside) contents were developed using modified partial least squares regression with internal cross-validation (n = 63). The equations obtained had low standard errors of cross-validation and moderate R2 (coefficient of determination in calibration). The prediction of an external validation set (n = 30) showed significant correlation between reference values and NIRS predicted values based on the SEP (standard error of prediction), bias, and r2 (coefficient of determination in prediction). The models developed in this study had relatively higher values (more than 2.0) of SD/SEP(C) for all lignans and lignan glycosides except for sesaminol diglucoside, which had a minor amount, indicating good correlation between the reference and the NIRS estimate. The results showed that NIRS, a nondestructive screening method, could be used to rapidly determine lignan and lignan glycoside contents in the breeding programs for high quality sesame.
芝麻（Sesamum indicum L.）含有丰富的木脂素，包括脂溶性木脂素（芝麻素和芝麻林素）以及与抗氧化活性相关的水溶性木脂素糖苷（芝麻素醇三糖苷和芝麻素醇二糖苷）。在本研究中，采用近红外反射光谱法（NIRS）开发了一种快速、无损测定完整芝麻种子中木脂素含量的方法。对93颗完整种子以扫描单色仪的反射模式进行扫描。该扫描过程无需对样品进行粉碎，每次分析可在数分钟内完成。通过高效液相色谱分析获得木脂素含量的参考值。使用带有内部交叉验证（n = 63）的改进偏最小二乘回归法建立了木脂素（芝麻素和芝麻林素）以及木脂素糖苷（芝麻素醇三糖苷和芝麻素醇二糖苷）含量的校准方程。所得到的方程具有较低的交叉验证标准误差和适中的R2（校准中的决定系数）。外部验证集（n = 30）的预测结果表明，基于SEP（预测标准误差）、偏差和r2（预测中的决定系数），参考值与NIRS预测值之间存在显著相关性。本研究中建立的模型，除含量较少的芝麻素醇二糖苷外，所有木脂素和木脂素糖苷的SD/SEP(C)值相对较高（大于2.0），表明参考值与NIRS估计值之间具有良好的相关性。结果表明，NIRS这种无损筛选方法可用于在高品质芝麻育种计划中快速测定木脂素和木脂素糖苷的含量。
