Graduate Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
J Agric Food Chem. 2010 Jan 13;58(1):563-9. doi: 10.1021/jf9028046.
Lignan glycosides are important functional compounds in sesame meal. In the present study, we investigated whether the tissue distribution of nano/submicrosized lignan glycosides from sesame meal (N-LGSM) differs from lignan glycosides from sesame meal (LGSM). LGSM was nano/submicrosized with 0.3 mm zirconia beads as the milling media. The average particle size of the 4% LGSM aqueous suspension reduced rapidly from approximately 2 microm to 200 nm after media milling at an agitation speed of 3600 rpm for 30 min. We examined the tissue distribution of sesaminol triglucoside (ST), the main component in LGSM, in Sprague-Dawley (SD) rats. The concentrations of ST were determined in various tissues and plasma within a 24 h period after oral administration of N-LGSM and LGSM (800 mg/kg of body weight). The results showed that higher concentrations of ST and its metabolites (sesaminol, sesaminol sulfate, and sesaminol glucuronide) were found in N-LGSM compared to those in LGSM in most tissues, especially liver and small intestine. Sesaminol glucuronide was the main metabolite in rats. After 3 h of oral administration, around 70% higher concentration of sesaminol glucuronide was found in N-LGSM compared to that in LGSM. This study clearly showed that LGSM is more bioavailable after nano/submicrosizing.
芝麻中的木脂素糖苷是一种重要的功能性化合物。在本研究中，我们研究了芝麻中的纳米/亚微米级木脂素糖苷（N-LGSM）与芝麻木脂素糖苷（LGSM）在组织分布上是否存在差异。LGSM 经 0.3mm 氧化锆珠作为研磨介质纳米/亚微米化。在搅拌速度为 3600rpm 的条件下研磨 30min 后，4%LGSM 水悬浮液的平均粒径迅速从约 2μm减小到 200nm。我们考察了主成分芝麻素三葡萄糖苷（ST）在 LGSM 中的组织分布，在 SD 大鼠中进行了口服 N-LGSM 和 LGSM（800mg/kg 体重）后 24h 内各组织和血浆中 ST 的浓度检测。结果表明，与 LGSM 相比，N-LGSM 在大多数组织，特别是肝脏和小肠中，ST 及其代谢物（芝麻素、芝麻素硫酸盐和芝麻素葡萄糖苷）的浓度更高。在大鼠体内，芝麻素葡萄糖苷是主要代谢物。口服 3h 后，N-LGSM 中芝麻素葡萄糖苷的浓度比 LGSM 高约 70%。本研究清楚地表明，LGSM 纳米/亚微米化后生物利用度更高。
