Cohen M H, Drage D J, Robertson A
Biophys J. 1975 Aug;15(8):753-63. doi: 10.1016/S0006-3495(75)85852-8.
The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.
本文描述了一种可控量环磷酸腺苷（c - AMP）源的设计、校准和操作。通常，可将含有10¹⁰ - 10⁻¹²个c - AMP分子的1.5秒脉冲输送到琼脂平板上直径约10微米的区域。给出了所得浓度分布随距离和时间的函数关系。在21℃下，测得c - AMP在琼脂中的扩散系数为0.97×10⁻⁵平方厘米每秒。
