Robertson A, Drage D J
Biophys J. 1975 Aug;15(8):765-75. doi: 10.1016/S0006-3495(75)85853-X.
The microelectrode system described in the accompanying paper was used to investigate properties of fields of Dictyostelium discoideum amoebae in late interphase. Cells in the fields were competent to respond chemotactically to, and to relay, a c-AMP signal, but not to produce an aggregative signal autonomously. The experimental results are generally consistent with c-AMP being the sole compound required for chemotaxis and signal relaying. A periodic signal from the microelectrode can initiate and control aggregation and can complete with spontaneously arising aggregates. The electrode was used to measure the refractory period for relaying which decreases from 9 min or more to between 2 and 3 min with increasing developmental age, and to measure thresholds for chemotaxis and signal relaying. The results are discussed in relation to models for the control of aggregation in D. discoideum.
随附论文中描述的微电极系统用于研究盘基网柄菌变形虫在分裂间期后期的场特性。场中的细胞能够对c - AMP信号作出趋化反应并进行信号传递，但不能自主产生聚集信号。实验结果总体上与c - AMP是趋化作用和信号传递所需的唯一化合物这一观点一致。来自微电极的周期性信号可以启动和控制聚集，并能与自发形成的聚集体竞争。该电极用于测量信号传递的不应期，随着发育年龄的增加，不应期从9分钟或更长时间减少到2至3分钟之间，还用于测量趋化作用和信号传递的阈值。结合盘基网柄菌聚集控制模型对结果进行了讨论。
