Woo Charlotte, Li Haisheng, Baatrup Anette, Krause Andrea, Kassem Moustapha, Bunger Cody, Lind Martin
Orthopaedic Research Laboratory, Aarhus University Hospital, Nørrebrogade 44, building 1A, DK-8000 Aarhus C, Denmark.
J Biomed Mater Res A. 2006 Dec 1;79(3):552-6. doi: 10.1002/jbm.a.30892.
Since its osteoinductive capacity has been established, demineralized bone matrix is considered a suitable alternative to bone autograft in the healing of osseous defects. The mechanisms of bone formation induction are still not fully understood. In this study we assessed the effects of a dispersion of bovine bone extract COLLOSS (BPE) with regard to proliferation and differentiation of a human mesenchymal stem cell line overexpressing human telomerase reverse transcriptase (hMSC-TERT). Proliferation rate was determined by (3)H-thymidine incorporation. The differentiation of hMSC-TERT cells to osteoblastic cells was assessed by means of measuring alkaline phosphatase activity and collagen synthesis in vitro. Both undifferentiated and osteoblast-differentiated hMSC-TERT cells were investigated for response to BPE. The metabolic responses to BPE were compared to unstimulated cells and cells stimulated with bovine collagen (COL). Undifferentiated hMSC-TERT cells responded to BPE with increased proliferation and decreased alkaline phosphatase activity. Osteoblastic differentiated hMSC-TERT cells had a diminished proliferative response and an increased alkaline phosphatase activity and collagen synthesis. Our study demonstrated significant metabolic effects of BPE on hMSC-TERT cells, which were highly dependant on the differentiated state of the cells.
由于其骨诱导能力已得到证实，脱矿骨基质被认为是骨缺损愈合中自体骨移植的合适替代物。骨形成诱导的机制仍未完全了解。在本研究中，我们评估了牛骨提取物COLLOSS（BPE）的分散体对过表达人端粒酶逆转录酶的人间充质干细胞系（hMSC-TERT）增殖和分化的影响。通过³H-胸腺嘧啶核苷掺入法测定增殖率。通过体外测量碱性磷酸酶活性和胶原蛋白合成来评估hMSC-TERT细胞向成骨细胞的分化。研究了未分化和已分化为成骨细胞的hMSC-TERT细胞对BPE的反应。将对BPE的代谢反应与未刺激的细胞以及用牛胶原蛋白（COL）刺激的细胞进行比较。未分化的hMSC-TERT细胞对BPE的反应是增殖增加和碱性磷酸酶活性降低。已分化为成骨细胞的hMSC-TERT细胞增殖反应减弱，碱性磷酸酶活性和胶原蛋白合成增加。我们的研究证明了BPE对hMSC-TERT细胞具有显著的代谢作用，这高度依赖于细胞的分化状态。
