Li Yu-Ming, Schilling Tatjana, Benisch Peggy, Zeck Sabine, Meissner-Weigl Jutta, Schneider Doris, Limbert Catarina, Seufert Jochen, Kassem Moustapha, Schütze Norbert, Jakob Franz, Ebert Regina
Orthopedic Center for Musculoskeletal Research, Orthopedic Department, University of Würzburg, Brettreichstrasse 11, D-97074 Würzburg, Germany.
Biochem Biophys Res Commun. 2007 Nov 9;363(1):209-15. doi: 10.1016/j.bbrc.2007.08.161. Epub 2007 Sep 5.
High glucose (HG) concentrations impair cellular functions and induce apoptosis. Exposition of mesenchymal stem cells (MSC) to HG was reported to reduce colony forming activity and induce premature senescence. We characterized the effects of HG on human MSC in vitro using telomerase-immortalized MSC (hMSC-TERT) and primary MSC (hMSC). HG (25mM) enhanced hMSC-TERT proliferation in long-term studies in contrast to hMSC where proliferation was unchanged. Thioredoxin-interacting protein, which is involved in apoptosis regulation, was stimulated by glucose in hMSC-TERT. However, apoptosis was not influenced by HG in both cell types. MSC treatment with HG favored osteogenic differentiation. MSC are resistant to HG toxicity, depending on the stemness of MSC. Proliferation and osteogenic differentiation are stimulated by HG. Effects of HG on the transient amplifying compartment of MSC may differ from those in mature cells. Further research is needed to unravel the molecular mechanisms of HG resistance of MSC.
高葡萄糖（HG）浓度会损害细胞功能并诱导细胞凋亡。据报道，间充质干细胞（MSC）暴露于HG会降低集落形成活性并诱导早衰。我们使用端粒酶永生化的MSC（hMSC-TERT）和原代MSC（hMSC）在体外表征了HG对人MSC的影响。与增殖未改变的hMSC相比，在长期研究中HG（25mM）增强了hMSC-TERT的增殖。参与细胞凋亡调节的硫氧还蛋白相互作用蛋白在hMSC-TERT中受到葡萄糖刺激。然而，两种细胞类型中的细胞凋亡均不受HG影响。用HG处理MSC有利于成骨分化。MSC对HG毒性具有抗性，这取决于MSC的干性。HG刺激增殖和成骨分化。HG对MSC瞬时扩增区室的影响可能与成熟细胞不同。需要进一步研究以阐明MSC对HG抗性的分子机制。
