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大蚊虹彩病毒的物理化学性质

Physicochemical properties of tipula iridescent virus.

作者信息

Kalmakoff J, Tremaine J H

机构信息

Department of Microbiology, The University of British Columbia, and Canada Department of Agriculture Research Station, Vancouver, British Columbia, Canada.

出版信息

J Virol. 1968 Jul;2(7):738-44. doi: 10.1128/JVI.2.7.738-744.1968.

Abstract

The molecular weight of Tipula iridescent virus, based on sedimentation and diffusion coefficients, was 5.51 x 10(8), with hydration of 0.57 g of water per g of virus. Deoxyribonucleic acid content, based on total inorganic phosphorus liberated, was 19 +/- 0.2%. At 260 mmu, the virus gave an uncorrected absorbance of 18.2 cm(2)/mg of virus and a light-scattering corrected absorbance of 9.8 cm(2)/mg of virus. Amino acid analyses of the virus protein revealed a remarkable similarity to Sericesthis iridescent virus. The possibility is discussed that the four iridescent insect viruses reported to date bear a strain relationship.

摘要

基于沉降系数和扩散系数,大蚊虹彩病毒的分子量为5.51×10⁸,每克病毒的水合量为0.57克水。基于释放的总无机磷,脱氧核糖核酸含量为19±0.2%。在260毫微米处,该病毒的未校正吸光度为18.2平方厘米/毫克病毒,光散射校正吸光度为9.8平方厘米/毫克病毒。对该病毒蛋白质的氨基酸分析显示,它与丝蚕虹彩病毒有显著相似性。文中讨论了迄今为止报道的四种虹彩昆虫病毒存在株系关系的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa23/375682/de7d0544bdfe/jvirol00319-0088-a.jpg

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