125I标记和（3H）乙酸盐标记抗体检测虹彩病毒的比较研究
Comparative study of 125 I- and (3H)acetate-labeled antibodies in detecting iridescent viruses.
作者信息
Bilimoria S L, Parkinson A J, Kalmakoff J
出版信息
Appl Microbiol. 1974 Jul;28(1):133-7. doi: 10.1128/am.28.1.133-137.1974.
Radioimmunoassays for detecting cell-associated or released virus are described using either (125)I- or [(3)H]acetate-labeled antibodies. In the first assay system, antigen-antibody complexes were separated from free antibody by centrifugation. Sensitivities of 0.1 mug of iridescent virus could be achieved with either (125)I- or [(3)H]acetate-labeled antibody. In the second assay, the antigen was fixed to cover-slip cell cultures, and then reacted with labeled antibody, unbound radioactivity being removed by repeated washing. Nonspecific binding with this method was 0.5 to 1% of the total radioactivity added and sensitivities of 0.1 or 10 mug were achieved with (125)I and [(3)H]acetate, respectively. Immunoglobulins were labeled at the rate of 1 in 300 for (125)I and 1 in 200 with [(3)H]acetate although there was a 400-fold greater isotopic abundance of (125)I relative to (3)H. The possibility of preparing labeled protein of high specific activity using carrier-free [2-(3)H]iodoacetic acid is discussed.
描述了使用（125）I或[（3）H]乙酸盐标记的抗体来检测细胞相关或释放病毒的放射免疫测定法。在第一种测定系统中，通过离心将抗原-抗体复合物与游离抗体分离。使用（125）I或[（3）H]乙酸盐标记的抗体，可实现对0.1微克虹彩病毒的检测灵敏度。在第二种测定中，将抗原固定在盖玻片细胞培养物上，然后与标记抗体反应，通过反复洗涤去除未结合的放射性。用这种方法的非特异性结合为加入的总放射性的0.5%至1%，使用（125）I和[（3）H]乙酸盐分别实现了0.1或10微克的检测灵敏度。免疫球蛋白的标记率，（125）I为300分之一，[（3）H]乙酸盐为200分之一，尽管（125）I相对于（3）H的同位素丰度高400倍。讨论了使用无载体[2-（3）H]碘乙酸制备高比活性标记蛋白的可能性。
Zotero 插件