用于通过DNA探针检测肠道病原体的菌落印迹法和粪便印迹法的比较。
Comparison of colony and stool blots for detection of enteropathogens by DNA probes.
作者信息
Vieira M A, Guth B E, Gomes T A
机构信息
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brazil.
出版信息
Can J Microbiol. 1991 May;37(5):407-10. doi: 10.1139/m91-066.
DNA probes that identify genes coding for heat-labile type I (LT-I) and heat-stable type 1 (ST-I) enterotoxins, enteropathogenic Escherichia coli adherence factor (EAF), and Shigella-like invasiveness (INV) are used to evaluate the sensitivity and specificity of stool blots in comparison with the sensitivity and specificity of colony blots in detecting enteropathogens. The sensitivities of the probes in stool blots are 91.7% for the LT-I probe, 76.9% for the ST-I probes, 78.9% for the EAF probe, and 45.5% for the INV probe. The specificity of all probes is higher than 95%. In general, the stool blot method identifies as many if not more LT-I-, ST-I-, and EAF-producing E. coli infections than the colony blots.
用于鉴定编码不耐热I型（LT-I）和耐热1型（ST-I）肠毒素、肠致病性大肠杆菌黏附因子（EAF）以及志贺样侵袭性（INV）基因的DNA探针，被用于评估粪便印迹法与菌落印迹法在检测肠道病原体方面的敏感性和特异性。粪便印迹法中各探针的敏感性分别为：LT-I探针91.7%、ST-I探针76.9%、EAF探针78.9%、INV探针45.5%。所有探针的特异性均高于95%。总体而言，粪便印迹法鉴定出的产LT-I、ST-I和EAF的大肠杆菌感染数量即便不比菌落印迹法多，也与之相当。
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