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Use of probes to detect virulence factor DNA sequences in Escherichia coli strains isolated from foods.

作者信息

Franco B D, Gomes T A, Jakabi M, Marques L R

机构信息

Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.

出版信息

Int J Food Microbiol. 1991 Apr;12(4):333-8. doi: 10.1016/0168-1605(91)90147-h.

DOI:10.1016/0168-1605(91)90147-h
PMID:1677256
Abstract

Escherichia coli strains were isolated from 96 food samples (32 milks, 4 dairy products, 36 raw meats, 7 meat products, 7 sandwiches and 10 ready-to-eat meals). A total of 306 colonies was submitted to hybridization assays with DNA probes for the following virulence factors: heat-labile toxins (LT-I and LT-II), heat-stable toxins (ST-h and ST-p). Shiga-like toxins (SLT-I and SLT-II), adherence factor of enteropathogenic E. coli (EAF) and invasive factor (INV). Six colonies isolated from 4 food samples hybridized with the probes for LT-II (3 colonies isolated from a milk sample), SLT-I and SLT-II (1 colony isolated from raw bovine meat) or EAF (2 colonies isolated from two raw chicken meat samples).

摘要

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Type II heat-labile enterotoxins from 50 diverse Escherichia coli isolates belong almost exclusively to the LT-IIc family and may be prophage encoded.50 株不同的大肠杆菌分离株中的 II 型不耐热肠毒素几乎完全属于 LT-IIc 家族,可能由噬菌体编码。
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