Loperena Lyliam, Ferrari Mario Daniel, Saravia Verónica, Murro Daiman, Lima Cynthia, Ferrando Lucía, Fernández Ana, Lareo Claudia
Dept. Bioingeniería, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, J. Herrera y Reissig 565, 11300, Montevideo, Uruguay.
Bioresour Technol. 2007 Mar;98(5):1045-51. doi: 10.1016/j.biortech.2006.04.030. Epub 2006 Jun 21.
The effectiveness of a commercial inoculum for degrading a dairy wastewater with high fat content was evaluated, and compared with an activated sludge inoculum from a dairy wastewater treatment pond. Both inocula reached similar chemical oxygen demand removal in batch experiments. The population dynamics was also studied by determining heterotrophic counts. Predominant microorganisms were differentiated by colony morphology and genomic fingerprinting (BOX-PCR) analysis. The higher population diversity and the wider range of CO2 production rate observed in batch reactors inoculated with activated-sludge, indicated that microorganisms from this inoculum were well adapted and may have had synergic activity for the degradation of the dairy effluent. When the bioreactor was operated with the commercial inoculum in continuous mode, according to its microbial growth kinetics, other microorganisms became predominant. These results showed that inoculated microorganisms did not persist in the open system and periodic addition of microorganisms may be needed to achieve a high performance treatment.
评估了一种商业接种物对降解高脂肪含量的乳制品废水的有效性，并与来自乳制品废水处理池的活性污泥接种物进行了比较。在分批实验中，两种接种物的化学需氧量去除率相似。还通过测定异养菌数来研究种群动态。通过菌落形态和基因组指纹分析（BOX-PCR）区分优势微生物。在接种活性污泥的分批反应器中观察到较高的种群多样性和更广泛的二氧化碳产生速率，这表明该接种物中的微生物适应性良好，并且可能对乳制品废水的降解具有协同活性。当生物反应器以连续模式使用商业接种物运行时，根据其微生物生长动力学，其他微生物成为优势菌。这些结果表明，接种的微生物在开放系统中不能持续存在，可能需要定期添加微生物以实现高效处理。
