Loperena Lyliam, Ferrari Mario Daniel, Díaz Ana Laura, Ingold Guzmán, Pérez Leticia Verónica, Carvallo Francisco, Travers Dayana, Menes Rodolfo Javier, Lareo Claudia
Dept Bioingeniería, Instituto de Ingeniería Química, Facultad de Ingeniería, Universidad de la República, Montevideo, Uruguay.
Bioresour Technol. 2009 Mar;100(5):1762-6. doi: 10.1016/j.biortech.2008.09.056. Epub 2008 Nov 17.
Milk fat/protein degrading microorganisms were isolated from different locations of a dairy wastewater treatment system with the goal of developing an inoculum for bioaugmentation strategies. Eight isolates, identified by 16S rRNA gene sequence analysis as belonging to the genera Bacillus, Pseudomonas, and Acinetobacter, were tested for their ability to remove COD and protein from a milk-based medium (3000 mg/L COD) and compared to a commercial bioaugmentation inoculum. The Acinetobacter isolate exhibited a pellet-type growth in liquid culture, a property that could potentially aid in the separation of microbes and liquid phase following treatment. Based on the individual degradation capacity and growth behavior of the isolates, three microorganisms were further selected and tested together. This consortium exhibited a COD removal similar to the commercial inoculum (57% and 63%, respectively), but higher protein (consortium: 93%; commercial inoculum: 54%), and fat removals (consortium: 75%; commercial inoculum: 38%).
从乳制品废水处理系统的不同位置分离出了乳脂肪/蛋白质降解微生物，目的是开发一种用于生物强化策略的接种物。通过16S rRNA基因序列分析鉴定出8株菌株，它们分别属于芽孢杆菌属、假单胞菌属和不动杆菌属，测试了它们从以牛奶为基础的培养基（化学需氧量为3000mg/L）中去除化学需氧量和蛋白质的能力，并与一种商业生物强化接种物进行了比较。不动杆菌分离株在液体培养中呈现出颗粒状生长，这一特性可能有助于处理后微生物与液相的分离。根据分离株的个体降解能力和生长行为，进一步选择了三种微生物并进行联合测试。该混合菌群的化学需氧量去除率与商业接种物相似（分别为57%和63%），但蛋白质去除率更高（混合菌群：93%；商业接种物：54%），脂肪去除率也更高（混合菌群：75%；商业接种物：38%）。
