Hergenröder Roland, Grass Benedikt
ISAS-Institute for Analytical Sciences, Dortmund, Germany.
Methods Mol Biol. 2006;339:113-26. doi: 10.1385/1-59745-076-6:113.
Conductivity detection as a versatile detection technique for chip-based electrophoretic separation methods is described and the basic principles are discussed. The necessary electronic equipment and technologies to implement the detection electrodes on a microchip are presented. A difference between contact and contactless detection is made and the resulting advantages and problems are shown. Different analytical applications are listed and discussed that show the basic applicability of conductivity detection in capillary electrophoresis and in isotachophoresis, which may serve as a guideline for more specific developments in the future. Central to all applications is the choice of the buffer system. It determines not only the separation capability but also puts limits on the achievable dynamic detection range.
本文描述了电导率检测作为基于芯片的电泳分离方法的一种通用检测技术，并讨论了其基本原理。介绍了在微芯片上实现检测电极所需的电子设备和技术。区分了接触式检测和非接触式检测，并展示了由此产生的优点和问题。列出并讨论了不同的分析应用，这些应用展示了电导率检测在毛细管电泳和等速电泳中的基本适用性，可为未来更具体的发展提供指导。所有应用的核心是缓冲系统的选择。它不仅决定了分离能力，还对可实现的动态检测范围设置了限制。
