Vandaveer Walter R, Pasas-Farmer Stephanie A, Fischer David J, Frankenfeld Celeste N, Lunte Susan M
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66047, USA.
Electrophoresis. 2004 Nov;25(21-22):3528-49. doi: 10.1002/elps.200406115.
Significant progress in the development of miniaturized microfluidic systems has occurred since their inception over a decade ago. This is primarily due to the numerous advantages of microchip analysis, including the ability to analyze minute samples, speed of analysis, reduced cost and waste, and portability. This review focuses on recent developments in integrating electrochemical (EC) detection with microchip capillary electrophoresis (CE). These detection modes include amperometry, conductimetry, and potentiometry. EC detection is ideal for use with microchip CE systems because it can be easily miniaturized with no diminution in analytical performance. Advances in microchip format, electrode material and design, decoupling of the detector from the separation field, and integration of sample preparation, separation, and detection on-chip are discussed. Microchip CEEC applications for enzyme/immunoassays, clinical and environmental assays, as well as the detection of neurotransmitters are also described.
自十多年前微型微流控系统问世以来，其发展已取得显著进展。这主要归功于微芯片分析的诸多优势，包括能够分析微量样品、分析速度快、成本和废物减少以及便携性。本综述重点关注将电化学（EC）检测与微芯片毛细管电泳（CE）相结合的最新进展。这些检测模式包括安培法、电导法和电位法。EC检测非常适合与微芯片CE系统一起使用，因为它可以很容易地小型化，而不会降低分析性能。本文讨论了微芯片形式、电极材料和设计、检测器与分离场的去耦以及芯片上样品制备、分离和检测的集成方面的进展。还描述了微芯片CEEC在酶/免疫分析、临床和环境分析以及神经递质检测中的应用。
