Chow Andrea W
Microfluidics Research and Development, Caliper Life Sciences, Mountain View, CA, USA.
Methods Mol Biol. 2006;339:145-58. doi: 10.1385/1-59745-076-6:145.
This chapter describes the use of two types of commercialized microfluidic chips for protein separation, suitable for personal scale and high-throughput use. Compared with conventional approaches, such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and capillary electrophoresis (CE), these devices offer the advantages of faster separation times, better data reproducibility, greater ease of use, labor savings in quantitative analysis, and ease in data archiving and data sharing owing to the digital data format. With some simple precautions taken to keep bubbles and particulates out of the microchannels, Lab-on-a-Chip devices have been adopted by many researchers in protein processing, protein engineering, and proteomics research laboratories to increase their productivity.
本章介绍了两种用于蛋白质分离的商业化微流控芯片的使用方法，适用于个人规模和高通量应用。与传统方法，如十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和毛细管电泳（CE）相比，这些设备具有分离时间更快、数据重现性更好、使用更简便、定量分析节省人力以及由于数字数据格式便于数据存档和共享等优点。通过采取一些简单的预防措施防止气泡和颗粒进入微通道，芯片实验室设备已被蛋白质加工、蛋白质工程和蛋白质组学研究实验室的许多研究人员采用，以提高他们的工作效率。
