Fogarty Barbara A, Lacher Nathan A, Lunte Susan M
Life Sciences Interface, Tyndall Institute, Cork, Ireland.
Methods Mol Biol. 2006;339:159-86. doi: 10.1385/1-59745-076-6:159.
The development of analytical methodologies to elucidate mechanisms of peptide transport and metabolism is important for the understanding of disease states and the design of effective drug therapies. Interest in the use of microchip capillary electrophoresis (CE) devices for peptide analysis stems from the ability to perform fast, highly efficient separations combined with small sample volume requirements. Many of the separation modes developed on conventional systems, including electrochromatography, isoelectric focusing, and electrophoretic bioaffinity assays, have been demonstrated on microchip devices. Steps that include sample preparation and labeling can also be integrated onto the microchip platform. This chapter will discuss considerations for peptide analysis using microchip CE and will focus on different approaches to sample preparation, separation, and detection.
开发用于阐明肽转运和代谢机制的分析方法对于理解疾病状态和设计有效的药物治疗至关重要。对使用微芯片毛细管电泳（CE）设备进行肽分析的兴趣源于其能够实现快速、高效的分离，同时所需样品体积小。在传统系统上开发的许多分离模式，包括电色谱、等电聚焦和电泳生物亲和测定，已在微芯片设备上得到验证。包括样品制备和标记在内的步骤也可以集成到微芯片平台上。本章将讨论使用微芯片CE进行肽分析的注意事项，并将重点介绍样品制备、分离和检测的不同方法。
