Terpe Kay
IBA GmbH, 37079, Göttingen, Germany.
Appl Microbiol Biotechnol. 2006 Sep;72(2):211-22. doi: 10.1007/s00253-006-0465-8. Epub 2006 Jun 22.
During the proteomics period, the growth in the use of recombinant proteins has increased greatly in the recent years. Bacterial systems remain most attractive due to low cost, high productivity, and rapid use. However, the rational choice of the adequate promoter system and host for a specific protein of interest remains difficult. This review gives an overview of the most commonly used systems: As hosts, Bacillus brevis, Bacillus megaterium, Bacillus subtilis, Caulobacter crescentus, other strains, and, most importantly, Escherichia coli BL21 and E. coli K12 and their derivatives are presented. On the promoter side, the main features of the l-arabinose inducible araBAD promoter (PBAD), the lac promoter, the l-rhamnose inducible rhaP BAD promoter, the T7 RNA polymerase promoter, the trc and tac promoter, the lambda phage promoter p L , and the anhydrotetracycline-inducible tetA promoter/operator are summarized.
在蛋白质组学时代，近年来重组蛋白的使用量大幅增长。由于成本低、生产率高且使用迅速，细菌系统仍然最具吸引力。然而，为特定的目标蛋白合理选择合适的启动子系统和宿主仍然很困难。本综述概述了最常用的系统：作为宿主，介绍了短短芽孢杆菌、巨大芽孢杆菌、枯草芽孢杆菌、新月柄杆菌、其他菌株，以及最重要的大肠杆菌BL21和大肠杆菌K12及其衍生物。在启动子方面，总结了L-阿拉伯糖诱导型araBAD启动子（PBAD）、乳糖启动子、L-鼠李糖诱导型rhaP BAD启动子、T7 RNA聚合酶启动子、trc和tac启动子、λ噬菌体启动子pL以及脱水四环素诱导型tetA启动子/操纵子的主要特征。
